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MCMBP antibody

Anti-MCMBP antibody used in Western Blot (WB). GTX120103

Cat. No. GTX120103

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MCMBP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCMBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885465

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex binding protein , C10orf119 , MCM-BP

Background

DNA replication is a carefully orchestrated process involving many proteins that assemble at origins of replication. Among these are the 6 proteins of the minichromosome maintenance (MCM) complex (e.g., MCM2; MIM 116945), which form a hexamer. Each MCM subunit performs an essential function in initiation and elongation of DNA replication. MCMBP can replace MCM2 in the MCM complex, thus forming an alternative MCM hexamer (Sakwe et al., 2007 [PubMed 17296731]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MCMBP antibody used in Western Blot (WB). GTX120103

GTX120103 WB Image

C10orf119 antibody detects C10orf119 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C10orf119 antibody (GTX120103) diluted by 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169