APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The antibody will neutralize the biological activity of recombinant human MCP-1.This antibody does not exhibit cross-reactivity with recombinant human RANTES, recombinant human MIP-1α, or recombinant mouse MIP-1α..
PROPERTIES
Form
Liquid
Buffer
PBS carbohydrates
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
recombinant human MCP-1/CCL2 expressed in E. coli.
Purification
Purified immunoglobulin
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-C motif chemokine ligand 2 , GDCF-2 , HC11 , HSMCR30 , MCAF , MCP-1 , MCP1 , SCYA2 , SMC-CF
Cellular Localization
Secreted
Background
Monocyte chemotactic protein (MCP1) is also known as monocyte chemotactic and activating factor (MCAF). It is the product of the human JE gene. The precursor form of MCP1 consists of 99 amino acids with a signal peptide sequence consisting of 23 amino-terminal acids. The mature form of MCP1 has 4 cysteine residues. The first two cysteine residues are in an adjacent position C-C, which characterizes MCP1 as a member of the chemokine beta subfamily. rhMCP1 is a non-glycosylated protein consisting of 76 amino acids with a molecular weight of 8.7 kDa. MCP1 mRNA expression can be induced in monocytes/macrophages, B lymphocytes, endothelial cells and astrocytoma cells by LPS. IL1 will induce production of MCP1 in fibroblasts, keratinocytes, hepatoma cells and Type II pneumocytes. In vitro, MCP1 will act on monocytes to initiate chemotaxis, induce superoxide anion release, induce the release of lysosomal enzymes, and augment cytostatic activity. In vivo, MCP1 will induce macrophage infiltration. The MCP1 gene contains potential binding sites for several transcription factors, including AP1, AP2, NFkB, and NFIL6.
Database
Research Area
REFERENCE
There are currently no references for MCP1 / CCL2 antibody [24822] (GTX10390). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCP1 / CCL2 antibody [24822] (GTX10390). Be the first to share your experience with this product.
