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MCP1 / CCL2 antibody [2D8]

Anti-MCP1 / CCL2 antibody [2D8] used in Flow cytometry (FACS). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in ELISA (ELISA). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60582

Cat No. GTX60582

Host

Mouse

Clonality

Monoclonal

Clone Name

2D8

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
IHC-Fr Assay dependent
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CCL2 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887982

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-C motif chemokine ligand 2 , GDCF-2 , HC11 , HSMCR30 , MCAF , MCP-1 , MCP1 , SCYA2 , SMC-CF

Cellular Localization

Secreted

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MCP1 / CCL2 antibody [2D8] used in Flow cytometry (FACS). GTX60582

GTX60582 FACS Image

FACS analysis of A549 cells using GTX60582 MCP1 / CCL2 antibody [2D8].
Green : MCP1 / CCL2
Red : negative control

Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582

GTX60582 WB Image

WB analysis of human CCL2 (AA: 1-99) recombinant protein using GTX60582 MCP1 / CCL2 antibody [2D8].

Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX60582

GTX60582 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60582 MCP1 / CCL2 antibody [2D8].

Anti-MCP1 / CCL2 antibody [2D8] used in ELISA (ELISA). GTX60582

GTX60582 ELISA Image

ELISA analysis of antigen using GTX60582 MCP1 / CCL2 antibody [2D8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582

GTX60582 WB Image

WB analysis of A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) using GTX60582 MCP1 / CCL2 antibody [2D8].

Anti-MCP1 / CCL2 antibody [2D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60582

GTX60582 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60582 MCP1 / CCL2 antibody [2D8].
Green : MCP1 / CCL2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

REVIEW

MCP1 / CCL2 antibody [2D8] Cat No. GTX60582

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Neuroinflammation in 2014. PMID: 25012628
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379