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MCP1 / CCL2 antibody, C-term

Anti-MCP1 / CCL2 antibody, C-term used in Western Blot (WB). GTX81767
Anti-MCP1 / CCL2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81767
Anti-MCP1 / CCL2 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81767
Anti-MCP1 / CCL2 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81767
Anti-MCP1 / CCL2 antibody, C-term used in Flow cytometry (FACS). GTX81767

Cat. No. GTX81767

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all MCP1 / CCL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Predict Reactivity

Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 62-89 amino acids from the C-terminal region of human CCL2.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11166975

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-C motif chemokine ligand 2 , GDCF-2 , HC11 , HSMCR30 , MCAF , MCP-1 , MCP1 , SCYA2 , SMC-CF

Cellular Localization

Secreted

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-MCP1 / CCL2 antibody, C-term used in Western Blot (WB). GTX81767

GTX81767 WB Image

WB analysis of HeLa cell lysate (35ug/lane) using GTX81767 MCP1 / CCL2 antibody, C-term.

Anti-MCP1 / CCL2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81767

GTX81767 IHC-P Image

IHC-P analysis of human brain tissue using GTX81767 MCP1 / CCL2 antibody, C-term.

Anti-MCP1 / CCL2 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81767

GTX81767 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX81767 MCP1 / CCL2 antibody, C-term.
Green : MCP1 / CCL2
Blue : DAPI

Anti-MCP1 / CCL2 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81767

GTX81767 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX81767 MCP1 / CCL2 antibody, C-term.
Green : CCL2
Red : Cell nuclear
Dilution : 0.025 mg/ml

Anti-MCP1 / CCL2 antibody, C-term used in Flow cytometry (FACS). GTX81767

GTX81767 FACS Image

FACS analysis of HeLa cells using GTX81767 MCP1 / CCL2 antibody, C-term.
Top histogram : negative control
Bottom histogram : HeLa cells

REFERENCE

Application Reference

Kaverina N et al. Autophagy 2017; : 43119 Astrocytes promote progression of breast cancer metastases to the brain via a KISS1-mediated autophagy.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399