GeneTex
United States (US)

MCP1 / CCL2 antibody [E10051]

GTX15784_316_ICC-IF_19011519_391.jpg
GTX15784_1536_WB_19011519_598.jpg

Cat No. GTX15784

Host Mouse
Clonality Monoclonal
Clone Name E10051
Isotype IgG1
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/mL
ICC/IF 2 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MCP-1

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-C Motif Chemokine Ligand 2,Gdcf-2,Hc11,Hsmcr30,Mcaf,Mcp-1,Mcp1,Scya2,Smc-Cf,Ccl2

Cellular Localization

Secreted

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES
GTX15784_316_ICC-IF_19011519_391.jpg

GTX15784 ICC/IF Image

ICC/IF analysis of HCT116 cells using GTX15784 MCP1 / CCL2 antibody [E10051]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature

GTX15784_1536_WB_19011519_598.jpg

GTX15784 WB Image

WB analysis of tissue extracts (30 µg lysate) of Mouse Pancreas (Lane 1), Spleen (Lane 2), Lung (Lane 3) and Cerebellum (Lane 4) using GTX15784 MCP1 / CCL2 antibody [E10051].
Dilution : 1-2 µg/ml