APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2 μg/mL |
| 2 μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.05 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human MCP-1
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-C motif chemokine ligand 2 , GDCF-2 , HC11 , HSMCR30 , MCAF , MCP-1 , MCP1 , SCYA2 , SMC-CF
Cellular Localization
Secreted
Background
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
![Anti-MCP1 / CCL2 antibody [E10051] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15784](/templates/default/js/lightbox/images/loading.gif "Anti-MCP1 / CCL2 antibody [E10051] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15784")
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GTX15784 ICC/IF Image
ICC/IF analysis of HCT116 cells using GTX15784 MCP1 / CCL2 antibody [E10051]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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GTX15784 WB Image
WB analysis of tissue extracts (30 μg lysate) of Mouse Pancreas (Lane 1), Spleen (Lane 2), Lung (Lane 3) and Cerebellum (Lane 4) using GTX15784 MCP1 / CCL2 antibody [E10051].
Dilution : 1-2 μg/ml
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REFERENCE
There are currently no references for MCP1 / CCL2 antibody [E10051] (GTX15784). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCP1 / CCL2 antibody [E10051] (GTX15784). Be the first to share your experience with this product.
![Anti-MCP1 / CCL2 antibody [E10051] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15784](/upload/website/prouct_img/small/GTX15784/GTX15784_316_ICC-IF_w_23060620_898.webp "Anti-MCP1 / CCL2 antibody [E10051] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15784")
![Anti-MCP1 / CCL2 antibody [E10051] used in Western Blot (WB). GTX15784](/upload/website/prouct_img/small/GTX15784/GTX15784_1536_WB_w_23060620_978.webp "Anti-MCP1 / CCL2 antibody [E10051] used in Western Blot (WB). GTX15784")
![Anti-MCP1 / CCL2 antibody [E10051] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15784](/upload/website/prouct_img/normal/GTX15784/GTX15784_316_ICC-IF_w_23060620_898.webp "Anti-MCP1 / CCL2 antibody [E10051] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15784")
![Anti-MCP1 / CCL2 antibody [E10051] used in Western Blot (WB). GTX15784](/upload/website/prouct_img/normal/GTX15784/GTX15784_1536_WB_w_23060620_978.webp "Anti-MCP1 / CCL2 antibody [E10051] used in Western Blot (WB). GTX15784")