APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.8 μg/ml |
Assay dependent |
Note :
WB
Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk.
IHC-Fr
Sections (6 μm) were fixed with 4% PFA, blocked using 0.1% Triton-X and 5% serum for 15min at 37ºC or o/n 4 ºC and then incubated with 2 μg/ml antibody for 2h at 37ºC.
|
Not tested in other applications.
Calculated MW
Product Note
We do not recommend use of this product for Human samples.
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic peptide corresponding to residues 102-130 of mouse MCP-1
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chemokine (C-C motif) ligand 2 , AI323594 , HC11 , JE , MCAF , MCP-1 , MCP1 , SMC-CF , Scya2 , Sigje
Cellular Localization
Secreted
Background
This gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. [provided by RefSeq, Sep 2015]
Database
Research Area
REFERENCE
There are currently no references for MCP1 / CCL2 antibody [ECE.2] (GTX54381). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCP1 / CCL2 antibody [ECE.2] (GTX54381). Be the first to share your experience with this product.