*Optimal dilutions/concentrations should be determined by the researcher.
Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk.
Sections (6 μm) were fixed with 4% PFA, blocked using 0.1% Triton-X and 5% serum for 15min at 37ºC or o/n 4 ºC and then incubated with 2 μg/ml antibody for 2h at 37ºC.
Not tested in other applications.
We do not recommend use of this product for Human samples.
0.2μm-filtered PBS, 0.1% BSA
0.02% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
100 μg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide corresponding to residues 102-130 of mouse MCP-1
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
chemokine (C-C motif) ligand 2 , AI323594 , HC11 , JE , MCAF , MCP-1 , MCP1 , SMC-CF , Scya2 , Sigje
This gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. [provided by RefSeq, Sep 2015]