ELISA: Use at an assay dependent dilution. Neut: Use at a concentration of 0.5 μg/ml. WB: Use at a concentration of 0.1 - 5 μg/ml. At a concentration of 0.1 - 1.0 μg/mL the antibody will allow visualization of 0.1 μg/lane of human MCP1. Concentration of 5.0 μg/mL will allow visualization of 50 μg/lane of rabbit MCP1. Predicted molecular weight: 11 kDa. Optimal dilutions/concentrations should be determined by the end user.
GTX18678 reacts with natural and recombinant human MCP1. It does not react with human interleukin 8 (IL8) and other human cytokines tested such as interleukin 1 beta (IL1 beta), serum amyloid A (SAA) and epidermal growth factor (EGF).
0.01M PBS pH7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant full length protein (Human).
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
C-C motif chemokine ligand 2 , GDCF-2 , HC11 , HSMCR30 , MCAF , MCP-1 , MCP1 , SCYA2 , SMC-CF
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]