APPLICATION
Application Note
For ELISA: Use at an assay dependent dilution. This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-1 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated or biotin-conjugated). Suggested Capture Coating Dose: 0.3μg/well; Substrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL of MCP-1 in serum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600 pg/mL. For Neut: Use at a concentration of 0.5 μg/ml. Tests on the ability to inhibit monocyte chemotaxis toward 1 nM recombinant human MCP-1 in blindwell chambers showed that the antibodies were particularly effective at blocking MCP-1 activity at a concentration of 0.5μg/mL. It was also found that S-14 could inhibit the function of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1. For WB: Use at a concentration of 0.1-1.0 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Product Note
This monoclonal antibody reacts with natural and recombinant human MCP-1. Does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1? (IL-1?), serum amyloid A (SAA) and epidermal growth factor (EGF).
PROPERTIES
Form
Liquid
Buffer
PBS pH7.2
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full length protein (Human).
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
C-C motif chemokine ligand 2 , GDCF-2 , HC11 , HSMCR30 , MCAF , MCP-1 , MCP1 , SCYA2 , SMC-CF
Cellular Localization
Secreted
Background
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Database
Research Area
REFERENCE
There are currently no references for MCP1 / CCL2 antibody [S14] (GTX18677). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCP1 / CCL2 antibody [S14] (GTX18677). Be the first to share your experience with this product.
