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MCP1 / CCL2 antibody

Anti-MCP1 / CCL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00641
Anti-MCP1 / CCL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00641
Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX00641
Anti-MCP1 / CCL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00641
Anti-MCP1 / CCL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00641
Anti-MCP1 / CCL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00641

Cat. No. GTX00641

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MCP1 / CCL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human MCP-1 (NP_002973.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CC motif chemokine ligand 2 , CCL2 , GDCF2 , HC11 , HSMCR30 , MCAF , MCP1 , SCYA2 , SMCCF , JE , C-C motif chemokine ligand 2

Cellular Localization

Secreted

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-MCP1 / CCL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00641

GTX00641 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX00641 MCP1 / CCL2 antibody.
Dilution : 1:100

Anti-MCP1 / CCL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00641

GTX00641 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX00641 MCP1 / CCL2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX00641

GTX00641 WB Image

WB analysis of various sample lysates using GTX00641 MCP1 / CCL2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-MCP1 / CCL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00641

GTX00641 IHC-P Image

IHC-P analysis of human colon tissue using GTX00641 MCP1 / CCL2 antibody.
Dilution : 1:100

Anti-MCP1 / CCL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00641

GTX00641 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX00641 MCP1 / CCL2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MCP1 / CCL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00641

GTX00641 IHC-P Image

IHC-P analysis of human stomach tissue using GTX00641 MCP1 / CCL2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399