GeneTex
United States (US)

MCP1 / CCL2 antibody

MCP1-antibody_WB_GTX29779-1_18121411_797.jpg

Cat No. GTX29779

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse, Rat
APPLICATION

Application Note

For WB: Use at a dilution of 1:1,500 - 1:2,500. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

16 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline, pH 7.2, containing 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant fragment - amino acids 1-73 of rat MCP-1 (N-terminal)

Purification

Protein A affinity purified
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-C Motif Chemokine Ligand 2,Mcp-1,Scya2,Sigje,Ccl2

Background

Rat MCP-1/JE (monocyte chemoattracctant protein-1) is an 148 amino acid CC chemokine with a NH2- terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library1. This rat MCP-1/JE is 49 amino acid longer than human MCP-1 at 3’- end. This 3’-end is a serine and threonine rich zone, which is probably responsible for the extensive O- glycosylation and which explains for the higher molecular weight (25 kDa)2. In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP- 1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.

Database

Research Area

DATA IMAGES
MCP1-antibody_WB_GTX29779-1_18121411_797.jpg

GTX29779 WB Image

Rat MCP-1 detected in RAW cells stimulated with LPS for 15 hrs.
1. Lysate
2. Supernatant