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MCP1 / CCL2 antibody

Orthogonal
Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813
Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813
Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813
Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813
Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813

Cat. No. GTX48813

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Mouse, Bovine
Package
50 μg ($399)
See all MCP1 / CCL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ELISA 1:4,500
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11169950

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chemokine (C-C motif) ligand 2 , MCP-1 , MCP-1A , MCP1 , MCP1A , SCYA2

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES

Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813

GTX48813 WB Image

Western blot using GeneTex's protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with GeneTex's anti-bovine CCL2 antibody . After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.

Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813

GTX48813 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 27507058

Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813

GTX48813 WB Image

Orthogonal

The data was published in the journal PLoS One in 2017. PMID: 29045486

Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813

GTX48813 WB Image

Orthogonal

The data was published in the journal PLoS One in 2017. PMID: 29045486

Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48813

GTX48813 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29045486

REFERENCE

Application Reference

Zera K et al. PLoS One 2017; 12 (10) : e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
Li Y et al. Oncotarget 2016; 7 (37) : 60446-60460 Chemoprevention of intestinal tumorigenesis by the natural dietary flavonoid myricetin in APCMin/+ mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399