APPLICATION
Application Note
ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 8.5 kDa. This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3μg/well; Substrate:TMB.A Western blot using an antibody concentration of 0.5-2μg/mL will allow visualization of 0.1μg/lane of human MCP-3. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Product Note
This monoclonal antibody reacts with natural and recombinant human MCP 3. It does not show any cross reaction with other human cytokines or growth factors tested such as IL 1 beta, IL 8, MCAF, SAA and EGF.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (Human).(M.W. 8.5 kDa containing 76 amino acid residues).
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-C motif chemokine ligand 7 , FIC , MARC , MCP-3 , MCP3 , NC28 , SCYA6 , SCYA7
Cellular Localization
Secreted
Background
This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for MCP3 antibody [542] (GTX18693). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCP3 antibody [542] (GTX18693). Be the first to share your experience with this product.