ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 8.5 kDa. This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3μg/well; Substrate:TMB.A Western blot using an antibody concentration of 0.5-2μg/mL will allow visualization of 0.1μg/lane of human MCP-3. Optimal dilutions/concentrations should be determined by the end user.
This monoclonal antibody reacts with natural and recombinant human MCP 3. It does not show any cross reaction with other human cytokines or growth factors tested such as IL 1 beta, IL 8, MCAF, SAA and EGF.
0.01M PBS pH7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant fragment (Human).(M.W. 8.5 kDa containing 76 amino acid residues).
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
C-C motif chemokine ligand 7 , FIC , MARC , MCP-3 , MCP3 , NC28 , SCYA6 , SCYA7
This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]