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MCP3 antibody [542]

Cat No. GTX18693

Host

Mouse

Clonality

Monoclonal

Clone Name

542

Isotype

IgG2a

Application

WB, ELISA

Reactivity

Human
Package
250 μg ($379)

APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 8.5 kDa. This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3μg/well; Substrate:TMB.A Western blot using an antibody concentration of 0.5-2μg/mL will allow visualization of 0.1μg/lane of human MCP-3. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

11 kDa. ( Note )

Product Note

This monoclonal antibody reacts with natural and recombinant human MCP 3. It does not show any cross reaction with other human cytokines or growth factors tested such as IL 1 beta, IL 8, MCAF, SAA and EGF.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (Human).(M.W. 8.5 kDa containing 76 amino acid residues).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-C motif chemokine ligand 7 , FIC , MARC , MCP-3 , MCP3 , NC28 , SCYA6 , SCYA7

Cellular Localization

Secreted

Background

This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 379
Custom Conjugations