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MCT1 antibody [GT14612]

Anti-MCT1 antibody [GT14612] used in IHC (Paraffin sections) (IHC-P). GTX631643
Anti-MCT1 antibody [GT14612] used in Western Blot (WB). GTX631643
Anti-MCT1 antibody [GT14612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631643

Cat. No. GTX631643

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT14612

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MCT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

This antibody was raised against human MCT1 Intracellular domain.

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human MCT1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888249

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 16 member 1 , HHF7 , MCT , MCT1 , MCT1D

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MCT1 antibody [GT14612] used in IHC (Paraffin sections) (IHC-P). GTX631643

GTX631643 IHC-P Image

MCT1 antibody [GT14612] detects MCT1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
MCT1 stained by MCT1 antibody [GT14612] (GTX631643) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MCT1 antibody [GT14612] used in Western Blot (WB). GTX631643

GTX631643 WB Image

Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCT1 antibody [GT14612] (GTX631643) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MCT1 antibody [GT14612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631643

GTX631643 ICC/IF Image

MCT1 antibody [GT14612] detects MCT1 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCT1 protein stained by MCT1 antibody [GT14612] (GTX631643) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Del Olmo T et al. EMBO Rep 2019; 20 (2) APEX2-mediated RAB proximity labeling identifies a role for RAB21 in clathrin-independent cargo sorting.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399
$ 169