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MCT1 antibody

Overexpression
Anti-MCT1 antibody used in Western Blot (WB). GTX129599
Anti-MCT1 antibody used in Western Blot (WB). GTX129599
Anti-MCT1 antibody used in Immunoprecipitation (IP). GTX129599

Cat. No. GTX129599

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MCT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , human MCT1-transfected 293T

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886048

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 16 member 1 , HHF7 , MCT , MCT1 , MCT1D

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MCT1 antibody used in Western Blot (WB). GTX129599

GTX129599 WB Image

Overexpression

MCT1 antibody detects MCT1 protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human MCT1-transfected 293T cells
7.5 % SDS-PAGE
MCT1 antibody (GTX129599) dilution: 1:5000

Anti-MCT1 antibody used in Western Blot (WB). GTX129599

GTX129599 WB Image

MCT1 antibody detects MCT1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCT1 antibody (GTX129599) diluted by 1:1000.

Anti-MCT1 antibody used in Immunoprecipitation (IP). GTX129599

GTX129599 IP Image

Immunoprecipitation of MCT1 protein from HepG2 whole cell extracts using 5 μg of MCT1 antibody (GTX129599).
Western blot analysis was performed using MCT1 antibody (GTX129599).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Jiang E et al. FASEB J 2019; : fj201802226R Tumoral microvesicle-activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169