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MD2 antibody [1A2E3]

Anti-MD2 antibody [1A2E3] used in IHC (Paraffin sections) (IHC-P). GTX85121
Anti-MD2 antibody [1A2E3] used in Western Blot (WB). GTX85121
Anti-MD2 antibody [1A2E3] used in IHC (Paraffin sections) (IHC-P). GTX85121
Anti-MD2 antibody [1A2E3] used in IHC (Paraffin sections) (IHC-P). GTX85121

Cat. No. GTX85121

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1A2E3

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all MD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Human Spleen Tissue Slide , MD-2 Recombinant Protein

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphocyte antigen 96 , ESOP-1 , MD-2 , MD2 , ly-96

Cellular Localization

Secreted

Background

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Database

Research Area

DATA IMAGES

Anti-MD2 antibody [1A2E3] used in IHC (Paraffin sections) (IHC-P). GTX85121

GTX85121 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85121 MD2 antibody [1A2E3].
Dilution : 20 μg/ml

Anti-MD2 antibody [1A2E3] used in Western Blot (WB). GTX85121

GTX85121 WB Image

WB analysis of (A) 25 and (B) 125 ng MD-2 recombinant protein using GTX85121 MD2 antibody [1A2E3].
Working concentration : 1 μg/ml

Anti-MD2 antibody [1A2E3] used in IHC (Paraffin sections) (IHC-P). GTX85121

GTX85121 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85121 MD2 antibody [1A2E3].
Dilution : 2 μg/ml

Anti-MD2 antibody [1A2E3] used in IHC (Paraffin sections) (IHC-P). GTX85121

GTX85121 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85121 MD2 antibody [1A2E3].
Working concentration : 2.5 μg/ml

REFERENCE

Application Reference

Li Z et al. ACS Chem Neurosci 2023; 14 (3) : 400-417 Immunohistochemical Localization of MD2, a Co-Receptor of TLR4, in the Adult Mouse Brain.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429