APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 μg/mL |
2.5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Spleen Tissue Slide , MD-2 Recombinant Protein
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
lymphocyte antigen 96 , ESOP-1 , MD-2 , MD2 , ly-96
Cellular Localization
Secreted
Background
MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
Database
Research Area
DATA IMAGES
|
GTX85122 IHC-P Image
IHC-P analysis of human spleen tissue using GTX85122 MD2 antibody [9F1B1]. Working concentration : 2.5 μg/ml
|
|
GTX85122 IHC-P Image
IHC-P analysis of human spleen tissue using GTX85122 MD2 antibody [9F1B1]. Working concentration : 20 μg/ml
|
|
GTX85122 WB Image
WB analysis of (A) 25 and (B) 125 ng MD-2 recombinant protein using GTX85122 MD2 antibody [9F1B1]. Working concentration : 1 μg/ml
|
REFERENCE
There are currently no references for MD2 antibody [9F1B1] (GTX85122). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MD2 antibody [9F1B1] (GTX85122). Be the first to share your experience with this product.