GeneTex

  • Country / Location Selection

United States (US)

MD2 antibody

Orthogonal
Anti-MD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85517
Anti-MD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85517
Anti-MD2 antibody used in Western Blot (WB). GTX85517
Anti-MD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85517
Anti-MD2 antibody used in Western Blot (WB). GTX85517
Anti-MD2 antibody used in Western Blot (WB). GTX85517
Anti-MD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85517
Anti-MD2 antibody used in Western Blot (WB). GTX85517
Anti-MD2 antibody used in Western Blot (WB). GTX85517
Anti-MD2 antibody used in Western Blot (WB). GTX85517

Cat. No. GTX85517

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all MD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
IHC-P 2 μg/mL
IHC-Fr Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Mouse Spleen Tissue Lysate(GTX27937) , Rat Spleen Tissue Lysate(GTX27945) , Human Spleen Tissue Slide

Product Note

This antibody is specific for MD-2 Internal

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MD-2 antibody was raised against a peptide corresponding to 13 amino acids near the center of human MD-2.The immunogen is located within the last 50 amino acids of MD-2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10721942

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphocyte antigen 96 , ESOP-1 , MD-2 , MD2 , ly-96

Cellular Localization

Secreted

Background

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) (1-3). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses (4). TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS (5,6). The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4 (7).

Database

Research Area

DATA IMAGES

Anti-MD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85517

GTX85517 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX85517 MD2 antibody.
Working concentration : 10 μg/ml

Anti-MD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85517

GTX85517 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85517 MD2 antibody.
Working concentration : 2.5 μg/ml

Anti-MD2 antibody used in Western Blot (WB). GTX85517

GTX85517 WB Image

WB analysis of human MD-2 recombinant protein lysates using GTX85517 MD2 antibody.
Dilution : 0.5 μg/mL (Lane 1), 1μg/mL (Lane 2), and 2μg/mL (Lane 3)
Loading : 30 ng of lysates per lane

Anti-MD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85517

GTX85517 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85517 MD2 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-MD2 antibody used in Western Blot (WB). GTX85517

GTX85517 WB Image

WB analysis of rat spleen and thymus tissue lysates using GTX85517 MD2 antibody.
Dilution : 1 μg/mL

Anti-MD2 antibody used in Western Blot (WB). GTX85517

GTX85517 WB Image

WB analysis of mouse spleen tissue lysates using GTX85517 MD2 antibody.
Dilution : 0.5 μg/mL (Lane 1) and 1μg/mL (Lane 2)
Loading : 15 μg of lysates per lane

Anti-MD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85517

GTX85517 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX85517 MD2 antibody.
Working concentration : 2 μg/ml

Anti-MD2 antibody used in Western Blot (WB). GTX85517

GTX85517 WB Image

WB analysis of rat spleen tissue lysates using GTX85517 MD2 antibody.
Dilution : 0.5 μg/mL (Lane 1) and 1μg/mL (Lane 2)
Loading : 15 μg of lysates per lane

Anti-MD2 antibody used in Western Blot (WB). GTX85517

GTX85517 WB Image

WB analysis of mouse tissue lysates using GTX85517 MD2 antibody.
Dilution : 1 μg/mL
Loading : 15 μg of lysates per lane

Anti-MD2 antibody used in Western Blot (WB). GTX85517

GTX85517 WB Image

WB analysis of Daudi cell lysate using GTX85517 MD2 antibody.
Dilution : 1 μg/mL
Loading : 15 μg

REFERENCE

Application Reference

Li Z et al. ACS Chem Neurosci 2023; 14 (3) : 400-417 Immunohistochemical Localization of MD2, a Co-Receptor of TLR4, in the Adult Mouse Brain.
Fan Z et al. Front Pharmacol 2022; 13 : 884821 Neuronal MD2 induces long-term mental impairments in septic mice by facilitating necroptosis and apoptosis.
Application : WB, IHC-Fr
Reactivity : Mouse
Zuo W et al. J Neuroinflammation 2021; 18 (1) : 204 MD2 contributes to the pathogenesis of perioperative neurocognitive disorder via the regulation of α5GABAA receptors in aged mice.
Application : WB
Reactivity : Mouse
Lee SC et al. PLoS One 2015; 10 (3) : e0120700 Protective effect of leaf essential oil from Cinnamomum osmophloeum Kanehira on endotoxin-induced intestinal injury in mice associated with suppressed local expression of molecules in the signaling pathways of TLR4 and NLRP3.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for MD2 antibody (GTX85517). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399