APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 - 4 μg/mL |
5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Lymphoid Tissue Tissue Slide(GTX21833) , Daudi Cell Lysate(GTX23951) , Human Lymph node Tissue Lysate(GTX29055) , Human Heart Tissue Slide
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the center of human MDA5.The immunogen is located within amino acids 330 - 380 of MDA5.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interferon induced with helicase C domain 1 , AGS7 , Hlcd , IDDM19 , MDA-5 , MDA5 , RLR-2 , SGMRT1
Cellular Localization
Cytoplasm,Nucleus
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012]
Database
Research Area
DATA IMAGES
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GTX31305 IHC-P Image
IHC-P analysis of human lymph node tissue using GTX31305 MDA5 antibody. Working concentration : 20 μg/ml
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GTX31305 WB Image
WB analysis of Daudi cell lysate using GTX31305 MDA5 antibody. Working concentration : (A) 1, (B) 2, and (C) 4 μg/ml
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GTX31305 IHC-P Image
IHC-P analysis of human lymph node tissue using GTX31305 MDA5 antibody. Working concentration : 5 μg/ml
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REFERENCE
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REVIEW
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