GeneTex
United States (US)

MDA5 antibody

GTX31305_2509_WB_20180221_18121410_290.jpg
GTX31305_908_IHC-P_20180221_18121410_544.jpg
GTX31305_110_IHC-P_20180221_18121410_628.jpg

Cat No. GTX31305

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 4 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

Human Lymphoid Tissue Tissue Slide(GTX21833) , Daudi Cell Lysate(GTX23951) , Human Lymph node Tissue Lysate(GTX29055) , Human Heart Tissue Slide
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the center of human MDA5.The immunogen is located within amino acids 330 - 380 of MDA5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interferon Induced With Helicase C Domain 1,Ags7,Hlcd,Iddm19,Mda-5,Mda5,Rlr-2,Sgmrt1,Ifih1

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES
GTX31305_2509_WB_20180221_18121410_290.jpg

GTX31305 WB Image

WB analysis of Daudi cell lysate using GTX31305 MDA5 antibody.
Working concentration : (A) 1, (B) 2, and (C) 4 μg/ml

GTX31305_908_IHC-P_20180221_18121410_544.jpg

GTX31305 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX31305 MDA5 antibody.
Working concentration : 5 μg/ml

GTX31305_110_IHC-P_20180221_18121410_628.jpg

GTX31305 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX31305 MDA5 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329