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MDA5 antibody

Anti-MDA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54300
Anti-MDA5 antibody used in Western Blot (WB). GTX54300
Anti-MDA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54300
Anti-MDA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54300

Cat No. GTX54300

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human IFIH1 (NP_071451.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interferon induced with helicase C domain 1 , AGS7 , Hlcd , IDDM19 , MDA-5 , MDA5 , RLR-2 , SGMRT1

Cellular Localization

Cytoplasm,Nucleus

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-MDA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54300

GTX54300 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX54300 MDA5 antibody.
Dilution : 1:200

Anti-MDA5 antibody used in Western Blot (WB). GTX54300

GTX54300 WB Image

WB analysis of various sample lysates using GTX54300 MDA5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-MDA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54300

GTX54300 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54300 MDA5 antibody.
Blue : DAPI

Anti-MDA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54300

GTX54300 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX54300 MDA5 antibody.
Dilution : 1:200

REFERENCE

There are currently no references for MDA5 antibody (GTX54300). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339