GeneTex
United States (US)

MDA5 antibody

GTX54300_20180530_IHC-P_18121410_140.jpg
GTX54300_20180530_IHC-P_1_18121410_783.jpg
GTX54300_20180530_ICCIF_18121410_970.jpg

Cat No. GTX54300

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

117 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-205 of human IFIH1 (NP_071451.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RLR2 Antibody , HLCD Antibody , IFIH1 Antibody , IDDM19 Antibody

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES
GTX54300_20180530_IHC-P_18121410_140.jpg

GTX54300 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX54300 MDA5 antibody.
Dilution : 1:200

GTX54300_20180530_IHC-P_1_18121410_783.jpg

GTX54300 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX54300 MDA5 antibody.
Dilution : 1:200

GTX54300_20180530_ICCIF_18121410_970.jpg

GTX54300 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX54300 MDA5 antibody.
Blue : DAPI

Package List Price ($)
$ 319