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MDC1 antibody [N2N3]

Orthogonal
Anti-MDC1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX102673
Anti-MDC1 antibody [N2N3] used in Western Blot (WB). GTX102673
Anti-MDC1 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102673
Anti-MDC1 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102673
Anti-MDC1 antibody [N2N3] used in Western Blot (WB). GTX102673

Cat. No. GTX102673

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MDC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

227 kDa. ( Note )

Positive Control

HeLa nucleus

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MDC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163015

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mediator of DNA damage checkpoint 1 , NFBD1

Cellular Localization

Nucleus

Background

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MDC1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX102673

GTX102673 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using MDC1(GTX102673) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MDC1 antibody [N2N3] used in Western Blot (WB). GTX102673

GTX102673 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa nucleus
5% SDS PAGE
GTX102673 diluted at 1:1000

Anti-MDC1 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102673

GTX102673 ICC/IF Image

Orthogonal

The data was published in the journal Nucleic Acids Res in 2019.PMID: 30668775

Anti-MDC1 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102673

GTX102673 ICC/IF Image

Orthogonal

The data was published in the journal Nucleic Acids Res in 2019.PMID: 30668775

Anti-MDC1 antibody [N2N3] used in Western Blot (WB). GTX102673

GTX102673 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28630472

REFERENCE

Application Reference

Burger K et al. Nucleic Acids Res 2019; (Epub) Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Application : ICC/IF
Reactivity : Human
Chen YJ et al. Sci Rep 2017; 7 (1) : 3842 SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169