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MDH1 antibody, C-term

Anti-MDH1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81955
Anti-MDH1 antibody, C-term used in Western Blot (WB). GTX81955
Anti-MDH1 antibody, C-term used in Flow cytometry (FACS). GTX81955

Cat. No. GTX81955

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all MDH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11162116

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malate dehydrogenase 1 , HEL-S-32 , MDH-s , MDHA , MGC:1375 , MOR2

Cellular Localization

Cytoplasm

Background

This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-MDH1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81955

GTX81955 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX81955 MDH1 antibody, C-term.

Anti-MDH1 antibody, C-term used in Western Blot (WB). GTX81955

GTX81955 WB Image

WB analysis of HL-60, THP-1 cell lysate (35ug/lane) using GTX81955 MDH1 antibody, C-term.

Anti-MDH1 antibody, C-term used in Flow cytometry (FACS). GTX81955

GTX81955 FACS Image

FACS analysis of HL-60 cells using GTX81955 MDH1 antibody, C-term.
Top histogram : negative control
Bottom histogram : HL-60 cells

REFERENCE

Application Reference

Wu YH et al. PLoS One 2013; 8 (8) : e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399