MDM2 antibody [HL5171]

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody [HL5171] (GTX645423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

MDM2 antibody [HL5171] detects MDM2 protein by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MDM2 stained by MDM2 antibody [HL5171] (GTX645423) diluted at 1:500.
Blue: Fluoroshield™ with DAPI (GTX30920).