APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89355-PEP).
Product Note
This antibody is expected to recognize isoform MDM2 (NP_002383.2) only.
Predict Reactivity
Rat, Bovine, Cat, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-DELSGERQRKRHKSD, from the internal region of the protein sequence according to NP_002383.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MDM2 proto-oncogene , ACTFS , HDMX , hdm2
Cellular Localization
nucleoplasm , Cytoplasm , Nucleolus
Background
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
Database
Research Area
DATA IMAGES
|
GTX89355 WB Image
WB analysis of HEK293 overexpressing MDM2 (mock transfection in first lane) using GTX89355 MDM2 antibody, Internal.
|
|
GTX89355 WB Image
WB analysis of human liver lysate using GTX89355 MDM2 antibody, Internal. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for MDM2 antibody, Internal (GTX89355). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MDM2 antibody, Internal (GTX89355). Be the first to share your experience with this product.