GeneTex
United States (US)

MDM2 antibody [MD-219]

Cat No. GTX10344

Host Mouse
Clonality Monoclonal
Clone Name MD-219
Isotype IgG1
Application WB, ICC/IF, FACS, ELISA
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-2 μg/ml
ICC/IF Assay dependent
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Specificity/Sensitivity

The antibody shows predominantly nuclear and some cytoplasmic staining in cells.
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

recombinant mouse MDM2.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transformed Mouse 3T3 Cell Double Minute 2,1700007J15Rik,Aa415488,Mdm-2,Mdm2

Background

MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53-mediated transactivation, MDM2 also binds to p53 protein. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a key factor in human tumorigenesis. p53 can be subjected to negative regulation by the product of a single cellular protooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53. MDM2-p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing 5 additional splice variant transcripts from the full length MDM2 gene. The alternatively spliced transcripts tend to be expressed in tumorigenic tissue, whereas the full length MDM2 transcript is expressed in normal tissue.

Database

Research Area

Package List Price ($)
$ 289