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MDM2 antibody [SMP14]

Anti-MDM2 antibody [SMP14] used in Western Blot (WB). GTX70278
Anti-MDM2 antibody [SMP14] used in IHC (Paraffin sections) (IHC-P). GTX70278

Cat. No. GTX70278

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SMP14

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, IP, IHC (Resin sections)

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all MDM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1/200-1/500
IHC-Fr Assay dependent
IP Assay dependent
IHC (Resin sections) Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH8.0 is recommended for this purpose.
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HepG2 , HepG2 (starvation for 16 hr, and 1 μM Staurosporine treatment for 24 hr) ,

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372294

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MDM2 proto-oncogene , ACTFS , HDMX , hdm2

Cellular Localization

nucleoplasm , Cytoplasm , Nucleolus

Background

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-MDM2 antibody [SMP14] used in Western Blot (WB). GTX70278

GTX70278 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX70278) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Multiple bands were observed 60 ~ 97 kDa. It is possibly due to alternative splicing.
Reference:PMID: 9840926

Anti-MDM2 antibody [SMP14] used in IHC (Paraffin sections) (IHC-P). GTX70278

GTX70278 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX70278 MDM2 antibody [SMP14].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399