GeneTex
United States (US)

MDM2 antibody

Orthogonal Overexpression
GTX100531_42130_20170615_WB_B_19011506_137.jpg
GTX100531_40310_ChIP_19011506_630.jpg
GTX100531_42515_20160816_IHC-P_H_19011506_928.jpg
GTX100531_40310_WB_M_19011506_608.jpg
GTX100531_40310_IP_19011506_609.jpg
GTX100531_40310_IHC_19011506_959.jpg
GTX100531_43187_20180420_WB_treatment_Staurosporine_19011506_828.jpg
GTX100531_43187_20180824_WB_19011506_917.jpg

Cat No. GTX100531

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , HepG2 , HepG2 (starvation and 1 μM Staurosporine treatment for 24 hr) , MDM2-transfected 293T

Predict Reactivity

Rat, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mdm2 Proto-Oncogene,Actfs,Hdmx,Hdm2,Mdm2

Cellular Localization

Nucleus , nucleoplasm , Cytoplasm , nucleolus

Background

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100531_42130_20170615_WB_B_19011506_137.jpg

GTX100531 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100531_40310_ChIP_19011506_630.jpg

GTX100531 ChIP assay Image

Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.
ChIP experiment and primer designs are based on Nucleic Acids Res. 2005 Jan 7;33(1):13-26.

GTX100531_42515_20160816_IHC-P_H_19011506_928.jpg

GTX100531 IHC-P Image

MDM2 antibody detects MDM2 protein at cytoplasm in human lung by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
MDM2 antibody (GTX100531) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100531_40310_WB_M_19011506_608.jpg

GTX100531 WB Image

MDM2 antibody detects MDM2 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
MDM2 antibody (GTX100531) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100531_40310_IP_19011506_609.jpg

GTX100531 IP Image

MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 30 μg Jurkat cell whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MDM2 protein by 4 μg MDM2 antibody (GTX100531)
7.5 % SDS-PAGE
The immunoprecipitated MDM2 protein was detected by MDM2 antibody (GTX100531) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX100531_40310_IHC_19011506_959.jpg

GTX100531 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using MDM2(GTX100531) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100531_43187_20180420_WB_treatment_Staurosporine_19011506_828.jpg

GTX100531 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100531_43187_20180824_WB_19011506_917.jpg

GTX100531 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169