MDM2 antibody

Name: MDM2 antibody
Brand: GeneTex
SKU: GTX100531
Price: 419.00 USD
Rating: 5 (1 reviews)

Anti-MDM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100531

Anti-MDM2 antibody used in Western Blot (WB). GTX100531

Anti-MDM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100531

Anti-MDM2 antibody used in Immunoprecipitation (IP). GTX100531

Anti-MDM2 antibody used in ChIP assay (ChIP assay). GTX100531

Cat. No. GTX100531

Reference 9

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity	Human, Mouse
Package	100 μl ($419), 25 μl ($169)

See all MDM2 products

View product citations for antibody GTX100531 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HepG2 (starvation and 1 μM Staurosporine treatment for 24 hr)

Predict Reactivity

Rat, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624270

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MDM2 proto-oncogene , ACTFS , HDMX , hdm2

Cellular Localization

Nucleus , nucleoplasm , Cytoplasm , nucleolus

Background

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq]

Database

Gene ID: 4193 MDM2
UniProt: Q00987 MDM2

Research Area

DATA IMAGES

GTX100531 ICC/IF Image

MDM2 antibody detects MDM2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

GTX100531 WB Image

Untreated (–) and treated (+) HCT116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100531 IHC-P Image

MDM2 antibody detects MDM2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100531 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using MDM2(GTX100531) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100531 IP Image

MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 30 μg Jurkat cell whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MDM2 protein by 4 μg MDM2 antibody (GTX100531)
7.5 % SDS-PAGE
The immunoprecipitated MDM2 protein was detected by MDM2 antibody (GTX100531) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX100531 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100531 IHC-P Image

MDM2 antibody detects MDM2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100531 ChIP assay Image

Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.
ChIP experiment and primer designs are based on Nucleic Acids Res. 2005 Jan 7;33(1):13-26.

GTX100531 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.