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MDMX antibody [2D10F4]

Anti-MDMX antibody [2D10F4] used in IHC (Paraffin sections) (IHC-P). GTX83335
Anti-MDMX antibody [2D10F4] used in IHC (Paraffin sections) (IHC-P). GTX83335
Anti-MDMX antibody [2D10F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83335
Anti-MDMX antibody [2D10F4] used in Western Blot (WB). GTX83335

Cat. No. GTX83335

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D10F4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all MDMX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MDM4 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MDM4 regulator of p53 , HDMX , MDMX , MRP1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-MDMX antibody [2D10F4] used in IHC (Paraffin sections) (IHC-P). GTX83335

GTX83335 IHC-P Image

IHC-P analysis of human cerebra (left) and lung carcinoma (right) tissue using GTX83335 MDMX antibody [2D10F4].

Anti-MDMX antibody [2D10F4] used in IHC (Paraffin sections) (IHC-P). GTX83335

GTX83335 IHC-P Image

IHC-P analysis of human Tonsil tissue using GTX83335 MDMX antibody [2D10F4].

Anti-MDMX antibody [2D10F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83335

GTX83335 ICC/IF Image

ICC/IF analysis of HeLa (left) and L-02 (right) cells using GTX83335 MDMX antibody [2D10F4].
Green : MDMX
Red: Actin filaments

Anti-MDMX antibody [2D10F4] used in Western Blot (WB). GTX83335

GTX83335 WB Image

WB analysis of HeLa (1), A549 (2) and A431 (3) cell lysate using GTX83335 MDMX antibody [2D10F4].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399