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MDMX (phospho Ser367) antibody [#15]

Orthogonal
Anti-MDMX (phospho Ser367) antibody [#15] used in Western Blot (WB). GTX00677

Cat. No. GTX00677

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 #15

Isotype

 IgG2b

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all MDMX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB ~1 μg/ml
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.4), 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence of human Mdmx protein surrounding phospho-Ser367

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MDM4 regulator of p53 , HDMX , MDMX , MRP1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-MDMX (phospho Ser367) antibody [#15] used in Western Blot (WB). GTX00677

GTX00677 WB Image

Orthogonal

WB analysis of MCF cells with DNA damage using GTX00677 MDMX (phospho Ser367) antibody [#15]. MCF cells were preincubated with the proteasome inhibitor MG132 (20 μM) followed by exposed to DNA damaging agents adriamycin (3 μM) or etoposide (20 μM). The cell lysates were used for immunoprecipitation with anti-MdmX antibody and then analyzed by Western blotting.Induction of S367 phosphorylation after DNA damage is associated with increased binding of 14-3-3 to MdmX and accelerated MdmX degradation.

REFERENCE

Clone Reference

Okamoto K et al. Mol Cell Biol. 2005; 25 (21):9608-20 DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation.
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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399