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ME1 antibody [GT15611]

KO/KD
Anti-ME1 antibody [GT15611] used in Western Blot (WB). GTX632190
Anti-ME1 antibody [GT15611] used in IHC (Paraffin sections) (IHC-P). GTX632190
Anti-ME1 antibody [GT15611] used in IHC (Frozen sections) (IHC-Fr). GTX632190
Anti-ME1 antibody [GT15611] used in IHC (Paraffin sections) (IHC-P). GTX632190
Anti-ME1 antibody [GT15611] used in Western Blot (WB). GTX632190

Cat. No. GTX632190

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT15611

Isotype

 IgG2a

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ME1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Rat, Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888296

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malic enzyme 1 , HUMNDME , MES

Cellular Localization

Cytoplasm

Background

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ME1 antibody [GT15611] used in Western Blot (WB). GTX632190

GTX632190 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [GT15611] (GTX632190) diluted at 1:1000.

Anti-ME1 antibody [GT15611] used in IHC (Paraffin sections) (IHC-P). GTX632190

GTX632190 IHC-P Image

ME1 antibody [GT15611] detects ME1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ME1 antibody [GT15611] (GTX632190) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ME1 antibody [GT15611] used in IHC (Frozen sections) (IHC-Fr). GTX632190

GTX632190 IHC-Fr Image

Double-labeled immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: Vimentin antibody (GTX100619) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: ME1 antibody [GT15611] (GTX632190) diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ME1 antibody [GT15611] used in IHC (Paraffin sections) (IHC-P). GTX632190

GTX632190 IHC-P Image

ME1 antibody [GT15611] detects ME1 protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ME1 antibody [GT15611] (GTX632190) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ME1 antibody [GT15611] used in Western Blot (WB). GTX632190

GTX632190 WB Image

ME1 antibody detects ME1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody (GTX632190) diluted at a dilution of 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations