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ME1 antibody

KO/KD
Anti-ME1 antibody used in Western Blot (WB). GTX104122
Anti-ME1 antibody used in Western Blot (WB). GTX104122
Anti-ME1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104122
Anti-ME1 antibody used in Immunoprecipitation (IP). GTX104122
Anti-ME1 antibody used in Western Blot (WB). GTX104122
Anti-ME1 antibody used in Western Blot (WB). GTX104122
Anti-ME1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104122

Cat. No. GTX104122

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ME1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

PC-12 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950905

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malic enzyme 1 , HUMNDME , MES

Cellular Localization

Cytoplasm

Background

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ME1 antibody used in Western Blot (WB). GTX104122

GTX104122 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody (GTX104122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ME1 antibody used in Western Blot (WB). GTX104122

GTX104122 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX104122 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ME1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104122

GTX104122 IHC-P Image

ME1 antibody detects ME1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
ME1 antibody (GTX104122) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ME1 antibody used in Immunoprecipitation (IP). GTX104122

GTX104122 IP Image

Immunoprecipitation of ME1 protein from HeLa whole cell extracts using 5 μg of ME1 antibody (GTX104122).
Western blot analysis was performed using ME1 antibody (GTX104122).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ME1 antibody used in Western Blot (WB). GTX104122

GTX104122 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX104122 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ME1 antibody used in Western Blot (WB). GTX104122

GTX104122 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody (GTX104122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ME1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104122

GTX104122 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ME1(GTX104122) antibody at 1:200 dilution.

REFERENCE

Application Reference

Igelmann S et al. Mol Cell 2021; 81 (18) : 3848-3865.e19 A hydride transfer complex reprograms NAD metabolism and bypasses senescence.
Application : IHC-P
Reactivity : Mouse
Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
Choi EJ et al. Oncogene 2017; (Epub) A clinical drug library screen identifies * propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169