GeneTex
United States (US)

ME1 antibody

KO/KD
GTX104122_40632_20160721_WB_shRNA_watermark_19011506_453.jpg
GTX104122_39918_IHC_19011506_458.jpg
GTX104122_IFA_19011506_733.jpg
GTX104122_39918_WB_R_19011506_116.jpg
GTX104122_40632_20150529_IP_19011506_123.jpg
GTX104122_39918_WB_M_19011506_971.jpg
GTX104122_40632_20150327_WB_19011506_285.jpg

Cat No. GTX104122

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12

Predict Reactivity

Sheep, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Malic Enzyme 1,Humndme,Mes,Me1

Cellular Localization

Cytoplasm

Background

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]

Database

DATA IMAGES
GTX104122_40632_20160721_WB_shRNA_watermark_19011506_453.jpg

GTX104122 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody (GTX104122) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104122_39918_IHC_19011506_458.jpg

GTX104122 IHC-P Image

ME1 antibody detects ME1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
ME1 antibody (GTX104122) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104122_IFA_19011506_733.jpg

GTX104122 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ME1(GTX104122) antibody at 1:200 dilution.

GTX104122_39918_WB_R_19011506_116.jpg

GTX104122 WB Image

Sample (30 µg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX104122 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104122_40632_20150529_IP_19011506_123.jpg

GTX104122 IP Image

Immunoprecipitation of ME1 protein from HeLa whole cell extracts using 5 μg of ME1 antibody (GTX104122).
Western blot analysis was performed using ME1 antibody (GTX104122).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104122_39918_WB_M_19011506_971.jpg

GTX104122 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX104122 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104122_40632_20150327_WB_19011506_285.jpg

GTX104122 WB Image

ME1 antibody detects ME1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody (GTX104122) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169