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ME2 antibody

Anti-ME2 antibody used in Immunoprecipitation (IP). GTX130673
Anti-ME2 antibody used in Western Blot (WB). GTX130673
Anti-ME2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130673

Cat. No. GTX130673

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ME2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ME2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886318

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malic enzyme 2 , ODS1

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ME2 antibody used in Immunoprecipitation (IP). GTX130673

GTX130673 IP Image

Immunoprecipitation of ME2 protein from THP-1 whole cell extracts using 5 μg of ME2 antibody (GTX130673).
Western blot analysis was performed using ME2 antibody (GTX130673) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ME2 antibody used in Western Blot (WB). GTX130673

GTX130673 WB Image

ME2 antibody detects ME2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME2 antibody (GTX130673) diluted at a dilution of 1:1000.

Anti-ME2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130673

GTX130673 IHC-P Image

ME2 antibody detects ME2 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
ME2 antibody (GTX130673) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169