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ME2 antibody

Anti-ME2 antibody used in Western Blot (WB). GTX31915
Anti-ME2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31915
Anti-ME2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31915

Cat. No. GTX31915

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all ME2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide

Product Note

ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2.The immunogen is located within amino acids 340 - 390 of ME2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malic enzyme 2 , ODS1

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES

Anti-ME2 antibody used in Western Blot (WB). GTX31915

GTX31915 WB Image

WB analysis of human spleen tissue lysate using GTX31915 ME2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-ME2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31915

GTX31915 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31915 ME2 antibody.
Working concentration : 20 μg/ml

Anti-ME2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31915

GTX31915 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31915 ME2 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399