GeneTex
United States (US)

ME2 antibody

ME2-antibody-GTX87553-IHC-1_18121411_289.jpg
ME2-antibody-GTX87553-WB-1_18121411_799.jpg

Cat No. GTX87553

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

65 kDa. ( Note )

Specificity/Sensitivity

ME2 Antibody detects endogenous levels of total ME2 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ME2.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Malic Enzyme 2,Ods1,Me2

Background

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
ME2-antibody-GTX87553-IHC-1_18121411_289.jpg

GTX87553 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using ME2 Antibody. The picture on the right is treated with the synthesized peptide.

ME2-antibody-GTX87553-WB-1_18121411_799.jpg

GTX87553 WB Image

Western blot analysis of extracts from RAW264.7/LOVO cells, using ME2 Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339