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ME2 antibody

Anti-ME2 antibody used in Western Blot (WB). GTX87553
Anti-ME2 antibody used in IHC (Paraffin sections) (IHC-P). GTX87553

Cat. No. GTX87553

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all ME2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ME2 (201-250).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ME2 , ODS1 , malic enzyme 2 , malic enzyme 2, NAD(+)-dependent, mitochondrial

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES

Anti-ME2 antibody used in Western Blot (WB). GTX87553

GTX87553 WB Image

WB analysis of RAW264.7 and LOVO cell lysates using GTX87553 ME2 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ME2 antibody used in IHC (Paraffin sections) (IHC-P). GTX87553

GTX87553 IHC-P Image

IHC-P analysis of human placenta tissue using GTX87553 ME2 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399