WB analysis of nuclear extracts (25 μg) from HeLa cells using GTX60358 MECP2 antibody - ChIP grade.
Dilution : 1:1,000

ELISA analysis of peptides used for immunization using GTX60358 MECP2 antibody - ChIP grade.

WB analysis of whole cell extracts (40 μg) from HeLa cells transfected with MeCP2 siRNA (lane 2) and from an untransfected control (lane 1) using GTX60358 MECP2 antibody - ChIP grade.
Dilution : 1:1,000

ChIP analysis of sheared chromatin from 10⁶ U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).