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MECP2 antibody - ChIP grade

Anti-MECP2 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60358
Anti-MECP2 antibody - ChIP grade used in Western Blot (WB). GTX60358
Anti-MECP2 antibody - ChIP grade used in ELISA (ELISA). GTX60358
Anti-MECP2 antibody - ChIP grade used in Western Blot (WB). GTX60358

Cat. No. GTX60358

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, ChIP assay

Reactivity

 Human
Package
50 μg ($429)
See all MECP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ELISA 1:1,000
ChIP assay 1-5 μg
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MeCP2 (Methyl-CpG-binding domain protein 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887961

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyl-CpG binding protein 2 , AUTSX3 , MRX16 , MRX79 , MRXS13 , MRXSL , PPMX , RS , RTS , RTT

Cellular Localization

Nucleus

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-MECP2 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60358

GTX60358 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-MECP2 antibody - ChIP grade used in Western Blot (WB). GTX60358

GTX60358 WB Image

WB analysis of whole cell extracts (40 μg) from HeLa cells transfected with MeCP2 siRNA (lane 2) and from an untransfected control (lane 1) using GTX60358 MECP2 antibody - ChIP grade.
Dilution : 1:1,000

Anti-MECP2 antibody - ChIP grade used in ELISA (ELISA). GTX60358

GTX60358 ELISA Image

ELISA analysis of peptides used for immunization using GTX60358 MECP2 antibody - ChIP grade.

Anti-MECP2 antibody - ChIP grade used in Western Blot (WB). GTX60358

GTX60358 WB Image

WB analysis of nuclear extracts (25 μg) from HeLa cells using GTX60358 MECP2 antibody - ChIP grade.
Dilution : 1:1,000

REFERENCE

Application Reference

Lee LC et al. Genes Brain Behav 2019; 18 (5) : e12559 Multiple epigenetic biomarkers for evaluation of students' academic performance.
Application : WB
Reactivity : Human
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Package List Price ($)
$ 429