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MECP2 antibody

Anti-MECP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX22828
Anti-MECP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22828
Anti-MECP2 antibody used in Western Blot (WB). GTX22828
Anti-MECP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22828
Anti-MECP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX22828
Anti-MECP2 antibody used in Western Blot (WB). GTX22828

Cat. No. GTX22828

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat, Drosophila, Primate
Package
50 μg ($399)
See all MECP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1500
ICC/IF 1:100-1:200
IHC-P 1:200-1:2000
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic Peptide: M(1) V A G M L G L R E E K S E D(15) C

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_372299

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyl CpG binding protein 2 , 1500041B07Rik , D630021H01Rik , Mbd5 , WBP10

Cellular Localization

Nucleus

Background

DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the Sin3 and histone deactylase protein families.

Database

Research Area

DATA IMAGES

Anti-MECP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX22828

GTX22828 IHC-P Image

IHC-P analysis of rat brain tissue using GTX22828 MECP2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:1000

Anti-MECP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22828

GTX22828 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22828 MECP2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4ºC

Anti-MECP2 antibody used in Western Blot (WB). GTX22828

GTX22828 WB Image

WB analysis of 40 μg of nuclear protein extracts from BEAS-2B in sample buffer using GTX22828 MECP2 antibody.
Dilution : 1:1500

Anti-MECP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22828

GTX22828 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX22828 MECP2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4ºC

Anti-MECP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX22828

GTX22828 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX22828 MECP2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:500

Anti-MECP2 antibody used in Western Blot (WB). GTX22828

GTX22828 WB Image

WB analysis of HeLa cell extract using GTX22828 MECP2 antibody.

REFERENCE

Application Reference

Lee LC et al. Mol Brain 2021; 14 (1) : 152 Association of CaMK2A and MeCP2 signaling pathways with cognitive ability in adolescents.
Application : WB
Reactivity : Human
Cotto B et al. Neurobiol Dis 2018; 117 : 28-41 Cocaine-mediated activation of microglia and microglial MeCP2 and BDNF production.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399