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MED13 antibody

Orthogonal
Anti-MED13 antibody used in Western Blot (WB). GTX129674
Anti-MED13 antibody used in Western Blot (WB). GTX129674
Anti-MED13 antibody used in Western Blot (WB). GTX129674

Cat. No. GTX129674

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MED13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

239 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Product Note

Knockdown/Knockout validation was supported by references (PMID:36220895)

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MED13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886069

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mediator complex subunit 13 , ARC250 , DRIP250 , HSPC221 , THRAP1 , TRAP240

Cellular Localization

Nucleus

Background

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MED13 antibody used in Western Blot (WB). GTX129674

GTX129674 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MED13 antibody (GTX129674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MED13 antibody used in Western Blot (WB). GTX129674

GTX129674 WB Image

Orthogonal

The data was published in the 2018 in Elife. PMID: 29809150

Anti-MED13 antibody used in Western Blot (WB). GTX129674

GTX129674 WB Image

The data was published in the 2018 in Elife. PMID: 29809150

REFERENCE

Application Reference

Dimitrova E et al. Nat Struct Mol Biol 2022; 29 (10) : 1000-1010 Distinct roles for CKM-Mediator in controlling Polycomb-dependent chromosomal interactions and priming genes for induction.
Feldmann A et al. Nucleic Acids Res 2020; (Epub) CDK-Mediator and FBXL19 prime developmental genes for activation by promoting atypical regulatory interactions.
Application : WB
Reactivity : Mouse
Dimitrova E et al. Elife 2018; 7 FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment.
Dimitrova E.A. et al. bioRxiv 2018; (Epub) FBXL19 recruits CDK-Mediator to CpG islands of developmental genes to prime them for activation during lineage commitment.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169