GeneTex
United States (US)

MED23 antibody

MED23-antibody-GTX87948-ICC_IF-1_18122102_158.jpg
MED23-antibody-GTX87948-IHC-1_18122102_649.jpg

Cat No. GTX87948

Host Rabbit
Clonality Polyclonal
Application ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

156 kDa. ( Note )

Specificity/Sensitivity

MED23 Antibody detects endogenous levels of total MED23 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MED23.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mediator Complex Subunit 23,Arc130,Crsp130,Crsp133,Crsp3,Drip130,Mrt18,Sur-2,Sur2,Med23

Background

Required for transcriptional activation subsequent to the assembly of the preinitiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.

Database

DATA IMAGES
MED23-antibody-GTX87948-ICC_IF-1_18122102_158.jpg

GTX87948 ICC/IF Image

Immunofluorescence analysis of A549 cells, using MED23 Antibody. The picture on the right is treated with the synthesized peptide.

MED23-antibody-GTX87948-IHC-1_18122102_649.jpg

GTX87948 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MED23 Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339