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MED25 antibody, N-term

Anti-MED25 antibody, N-term used in Western Blot (WB). GTX49103
Anti-MED25 antibody, N-term used in ChIP assay (ChIP assay). GTX49103

Cat No. GTX49103

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ChIP assay

Reactivity

Human
Package
50 μg ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

human heart

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human MED25

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mediator complex subunit 25 , ACID1 , ARC92 , BVSYS , CMT2B2 , P78 , PTOV2 , TCBAP0758

Cellular Localization

Nucleus

Background

This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-MED25 antibody, N-term used in Western Blot (WB). GTX49103

GTX49103 WB Image

WB analysis of human heart tissue using GTX49103 MED25 antibody at 0.2-1μg/ml.

Anti-MED25 antibody, N-term used in ChIP assay (ChIP assay). GTX49103

GTX49103 ChIP assay Image

ChIP analysis of HCT116 cells using GTX49103 MED25 antibody. Use 0.5μg antibody GTX49103 MED25 antibody for ChIP assay.Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
(+) : Add blocking peptide
(-) : no blocking peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf