GeneTex

  • Country / Location Selection

United States (US)

MED28 antibody

Anti-MED28 antibody used in Western Blot (WB). GTX85388
Anti-MED28 antibody used in IHC (Paraffin sections) (IHC-P). GTX85388

Cat. No. GTX85388

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all MED28 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MED28 antibody was raised against a 17 amino acid synthetic peptide from near the center of human MED28.The immunogen is located within amino acids 50 - 100 of MED28.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10724199

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mediator complex subunit 28 , 1500003D12Rik , EG1 , magicin

Cellular Localization

Nucleus,Cytoplasm,Membrane

Background

The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin’s tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.

Database

DATA IMAGES

Anti-MED28 antibody used in Western Blot (WB). GTX85388

GTX85388 WB Image

WB analysis of human brain tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85388 MED28 antibody.
Working concentration : 1 μg/ml

Anti-MED28 antibody used in IHC (Paraffin sections) (IHC-P). GTX85388

GTX85388 IHC-P Image

IHC-P analysis of human brain tissue using GTX85388 MED28 antibody.
Working concentration : 2.5 μg/ml

REFERENCE

Application Reference

Huang CY et al. J Cell Physiol 2016; (Epub) MED28 Regulates Epithelial-Mesenchymal Transition Through NFκB in Human Breast Cancer Cells.
Application : WB
Reactivity : Human
Chun-Yin Huang et al. Journal of Functional Foods 2015; 12 : 158–167 Resveratrol and pterostilbene regulate MED28 (magicin/EG1) expression and cell growth in MCF7 human breast cancer cells
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for MED28 antibody (GTX85388). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399