GeneTex
United States (US)

MED4 antibody [N1], N-term

GTX108541_WB_19011507_497.jpg
GTX108541_39883_IFA_19011507_113.jpg

Cat No. GTX108541

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MED4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mediator Complex Subunit 4,Arc36,Drip36,Hspc126,Trap36,Vdrip,Med4

Cellular Localization

Nucleus

Background

The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II (see MIM 180660)-transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (summary by Sato et al., 2004 [PubMed 15175163]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX108541_WB_19011507_497.jpg

GTX108541 WB Image

Sample (30 ug of whole cell lysate)
A: A549 12% SDS PAGE
GTX108541 diluted at 1:1000

GTX108541_39883_IFA_19011507_113.jpg

GTX108541 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using MED4(GTX108541) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169