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MED4 antibody

Anti-MED4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31355
Anti-MED4 antibody used in Western Blot (WB). GTX31355

Cat. No. GTX31355

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Human Testis Tissue Lysate(GTX15374) , Human Testis Tissue Slide(GTX21748)

Product Note

At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MED4 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MED4.The immunogen is located within the last 50 amino acids of MED4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mediator complex subunit 4 , ARC36 , DRIP36 , HSPC126 , TRAP36 , VDRIP

Cellular Localization

Nucleus

Background

This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-MED4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31355

GTX31355 IHC-P Image

IHC-P analysis of human testis tissue using GTX31355 MED4 antibody.
Working concentration : 10 μg/ml

Anti-MED4 antibody used in Western Blot (WB). GTX31355

GTX31355 WB Image

WB analysis of human testis tissue lysate using GTX31355 MED4 antibody.
Working concentration : 0.5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399