GeneTex
United States (US)

MEF2A antibody [C2C3], C-term

GTX101286_39834_IHC_R_19011506_270.jpg
GTX101286_39834_IHC_M_19011506_498.jpg
GTX101286_39834_IFA_19011506_496.jpg
GTX101286_WB_19011506_657.jpg
GTX101286_39834_20160114_WB_R_19011506_692.jpg

Cat No. GTX101286

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , PC-12 , Rat-2

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MEF2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myocyte Enhancer Factor 2A,Adcad1,Rsrfc4,Rsrfc9,Mef2,Mef2A

Cellular Localization

Nucleus

Background

The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX101286_39834_IHC_R_19011506_270.jpg

GTX101286 IHC-P Image

MEF2A antibody [C2C3], C-term detects MEF2A protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
MEF2A antibody [C2C3], C-term (GTX101286) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101286_39834_IHC_M_19011506_498.jpg

GTX101286 IHC-P Image

MEF2A antibody [C2C3], C-term detects MEF2A protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
MEF2A antibody [C2C3], C-term (GTX101286) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101286_39834_IFA_19011506_496.jpg

GTX101286 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MEF2A(GTX101286) antibody at 1:200 dilution.

GTX101286_WB_19011506_657.jpg

GTX101286 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX101286 diluted at 1:1000

GTX101286_39834_20160114_WB_R_19011506_692.jpg

GTX101286 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2A antibody [C2C3], C-term (GTX101286) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169