APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
H1299 , PC-12 , Rat-2
Predict Reactivity
Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human MEF2A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037400
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
myocyte enhancer factor 2A , ADCAD1 , RSRFC4 , RSRFC9 , mef2
Cellular Localization
Nucleus
Background
The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286](/templates/default/js/lightbox/images/loading.gif "Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286")
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GTX101286 WB Image
Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX101286 diluted at 1:1000
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GTX101286 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2A antibody [C2C3], C-term (GTX101286) diluted at 1:500.
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GTX101286 IHC-P Image
MEF2A antibody [C2C3], C-term detects MEF2A protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
MEF2A antibody [C2C3], C-term (GTX101286) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101286 IHC-P Image
MEF2A antibody [C2C3], C-term detects MEF2A protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
MEF2A antibody [C2C3], C-term (GTX101286) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101286 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MEF2A(GTX101286) antibody at 1:200 dilution.
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REFERENCE
There are currently no references for MEF2A antibody [C2C3], C-term (GTX101286). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MEF2A antibody [C2C3], C-term (GTX101286). Be the first to share your experience with this product.
![Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286](/upload/website/prouct_img/small/GTX101286/GTX101286_WB_w_23060100_696.webp "Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286")
![Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286](/upload/website/prouct_img/small/GTX101286/GTX101286_39834_20160114_WB_R_w_23060100_536.webp "Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286")
![Anti-MEF2A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101286](/upload/website/prouct_img/small/GTX101286/GTX101286_39834_IHC_M_w_23060100_569.webp "Anti-MEF2A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101286")
![Anti-MEF2A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101286](/upload/website/prouct_img/small/GTX101286/GTX101286_39834_IHC_R_w_23060100_561.webp "Anti-MEF2A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101286")
![Anti-MEF2A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101286](/upload/website/prouct_img/small/GTX101286/GTX101286_39834_IFA_w_23060100_357.webp "Anti-MEF2A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101286")
![Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286](/upload/website/prouct_img/normal/GTX101286/GTX101286_WB_w_23060100_696.webp "Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286")
![Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286](/upload/website/prouct_img/normal/GTX101286/GTX101286_39834_20160114_WB_R_w_23060100_536.webp "Anti-MEF2A antibody [C2C3], C-term used in Western Blot (WB). GTX101286")
![Anti-MEF2A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101286](/upload/website/prouct_img/normal/GTX101286/GTX101286_39834_IHC_M_w_23060100_569.webp "Anti-MEF2A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101286")
![Anti-MEF2A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101286](/upload/website/prouct_img/normal/GTX101286/GTX101286_39834_IHC_R_w_23060100_561.webp "Anti-MEF2A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101286")
![Anti-MEF2A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101286](/upload/website/prouct_img/normal/GTX101286/GTX101286_39834_IFA_w_23060100_357.webp "Anti-MEF2A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101286")