GeneTex
United States (US)

MEF2A antibody [N1N2], N-term

GTX101163_39904_20180703_IHC-WM_Z_19011506_497.jpg
GTX101163_IFA_19011506_690.jpg
GTX101163_WB_19011506_904.jpg

Cat No. GTX101163

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Wm
Reactivity Human, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MEF2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myocyte Enhancer Factor 2A,Adcad1,Rsrfc4,Rsrfc9,Mef2,Mef2A

Cellular Localization

Nucleus

Background

The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX101163_39904_20180703_IHC-WM_Z_19011506_497.jpg

GTX101163 IHC-Wm Image

MEF2A antibody [N1N2], N-term detects MEF2A protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: MEF2A stained by MEF2A antibody [N1N2], N-term (GTX101163) diluted at 1:100.

GTX101163_IFA_19011506_690.jpg

GTX101163 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MEF2A(GTX101163) antibody at 1:200 dilution.

GTX101163_WB_19011506_904.jpg

GTX101163 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX101163 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169