MEF2A (phospho Thr312) antibody

Cat. No. GTX50114

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX50114 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:100-1:200
IHC-P	1:50-1:100

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 312 (L-A-T(p)-P-V) derived from human MEF2A.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myocyte enhancer factor 2A , ADCAD1 , RSRFC4 , RSRFC9 , mef2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Database

Gene ID: 4205 MEF2A
UniProt: Q02078 MEF2A

Research Area

DATA IMAGES

GTX50114 WB Image

WB analysis of extracts from C2C12 cells treated with Anisomycin or calf intestinal phosphatase (CIP) using GTX50114 MEF2A (phospho Thr312) antibody.

GTX50114 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50114 MEF2A (phospho Thr312) antibody.

GTX50114 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50114 MEF2A (phospho Thr312) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty