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MEF2A (phospho Thr312) antibody

Anti-MEF2A (phospho Thr312) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79019
Anti-MEF2A (phospho Thr312) antibody used in IHC (Paraffin sections) (IHC-P). GTX79019

Cat. No. GTX79019

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100 - 1:200
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human MEF2A around the phosphorylation site of threonine 312 (L-A-Tp-P-V).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11173645

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADCAD1,MEF2A,RSRFC4,RSRFC9,mef2,myocyte enhancer factor 2A

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-MEF2A (phospho Thr312) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79019

GTX79019 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX79019 MEF2A (phospho Thr312) antibody.
Red : Primary antibody

Anti-MEF2A (phospho Thr312) antibody used in IHC (Paraffin sections) (IHC-P). GTX79019

GTX79019 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79019 MEF2A (phospho Thr312) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399