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MEF2C antibody [HL2811]

Recombinant antibody Orthogonal
Anti-MEF2C antibody [HL2811] used in IHC (Paraffin sections) (IHC-P). GTX639952
Anti-MEF2C antibody [HL2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639952
Anti-MEF2C antibody [HL2811] used in Western Blot (WB). GTX639952
Anti-MEF2C antibody [HL2811] used in Western Blot (WB). GTX639952
Anti-MEF2C antibody [HL2811] used in IHC (Paraffin sections) (IHC-P). GTX639952

Cat. No. GTX639952

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2811

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MEF2C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human MEF2C.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myocyte enhancer factor 2C , C5DELq14.3 , DEL5q14.3

Cellular Localization

Nucleus

Background

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe cognitive disability, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-MEF2C antibody [HL2811] used in IHC (Paraffin sections) (IHC-P). GTX639952

GTX639952 IHC-P Image

MEF2C antibody [HL2811] detects MEF2C protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MEF2C stained by MEF2C antibody [HL2811] (GTX639952) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEF2C antibody [HL2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639952

GTX639952 ICC/IF Image

MEF2C antibody [HL2811] detects MEF2C protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEF2C stained by MEF2C antibody [HL2811] (GTX639952) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-MEF2C antibody [HL2811] used in Western Blot (WB). GTX639952

GTX639952 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2C antibody [HL2811] (GTX639952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-MEF2C antibody [HL2811] used in Western Blot (WB). GTX639952

GTX639952 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2C antibody [HL2811] (GTX639952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEF2C antibody [HL2811] used in IHC (Paraffin sections) (IHC-P). GTX639952

GTX639952 IHC-P Image

MEF2C antibody [HL2811] detects MEF2C protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
MEF2C stained by MEF2C antibody [HL2811] (GTX639952) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations