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MEF2C antibody

Comparison Orthogonal
Anti-MEF2C antibody used in Western Blot (WB). GTX105433
Anti-MEF2C antibody used in Western Blot (WB). GTX105433
Anti-MEF2C antibody used in Western Blot (WB). GTX105433
Anti-MEF2C antibody used in Immunoprecipitation (IP). GTX105433
Anti-MEF2C antibody used in IHC (Frozen sections) (IHC-Fr). GTX105433
Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433
Anti-MEF2C antibody used in Western Blot (WB). GTX105433
Anti-MEF2C antibody used in Western Blot (WB). GTX105433
Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433
Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433
Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433
Anti-MEF2C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105433

Cat. No. GTX105433

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MEF2C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
IHC 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEF2C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173300

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myocyte enhancer factor 2C , C5DELq14.3 , DEL5q14.3

Cellular Localization

Nucleus

Background

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MEF2C antibody used in Western Blot (WB). GTX105433

GTX105433 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with MEF2C antibody (GTX105433) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-MEF2C antibody used in Western Blot (WB). GTX105433

GTX105433 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with MEF2C antibody (GTX105433) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-MEF2C antibody used in Western Blot (WB). GTX105433

GTX105433 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEF2C antibody (GTX105433) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEF2C antibody used in Immunoprecipitation (IP). GTX105433

GTX105433 IP Image

MEF2C antibody immunoprecipitates MEF2C protein in IP experiments.
IP samples: K562 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of MEF2C protein by 4 μg MEF2C antibody (GTX105433)
5 % SDS-PAGE
The immunoprecipitated MEF2C protein was detected by MEF2C antibody (GTX105433) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-MEF2C antibody used in IHC (Frozen sections) (IHC-Fr). GTX105433

GTX105433 IHC-Fr Image

MEF2C antibody detects MEF2C protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: MEF2C stained by MEF2C antibody (GTX105433) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433

GTX105433 IHC-P Image

MEF2C antibody detects MEF2C protein at nucleus on Saos2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Saos2 xenograft.
MEF2C antibody (GTX105433) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MEF2C antibody used in Western Blot (WB). GTX105433

GTX105433 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2C antibody (GTX105433) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEF2C antibody used in Western Blot (WB). GTX105433

GTX105433 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2C antibody (GTX105433) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433

GTX105433 IHC-P Image

MEF2C antibody detects MEF2C protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MEF2C stained by MEF2C antibody (GTX105433) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433

GTX105433 IHC-P Image

MEF2C antibody detects MEF2C protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MEF2C stained by MEF2C antibody (GTX105433) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433

GTX105433 IHC-P Image

MEF2C antibody detects MEF2C protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
MEF2C antibody (GTX105433) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MEF2C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105433

GTX105433 ICC/IF Image

MEF2C antibody detects MEF2C protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEF2C protein stained by MEF2C antibody (GTX105433) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE

Application Reference

Eagle K et al. Blood Cancer Discov 2022; 3 (5) : 394-409 Transcriptional Plasticity Drives Leukemia Immune Escape.
Shinichiro Takashima et al. Sci Rep 2021; 11 (1) : 1520 Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells.
Application : ICC/IF
Reactivity : Mouse
Wright A et al. FASEB J 2021; 35 (11) : e21955 Lysine methyltransferase 2D regulates muscle fiber size and muscle cell differentiation.
Application : WB
Reactivity : Mouse
Chia-Ming Lee et al. bioRxiv 2019; (Epub) Single cell transcriptomic analysis revealed long-lasting adverse effects of prenatal tamoxifen administration on neurogenesis in prenatal and adult brains.
Application : IHC
Zhang Z et al. Sci Rep 2019; 9 (1) : 6362 Ensuring expression of four core cardiogenic transcription factors enhances cardiac reprogramming.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169