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MEF2C antibody

Anti-MEF2C antibody used in IHC (Frozen sections) (IHC-Fr). GTX105433
Anti-MEF2C antibody used in Western Blot (WB). GTX105433
Anti-MEF2C antibody used in Immunoprecipitation (IP). GTX105433
Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433
Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433
Anti-MEF2C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105433

Cat No. GTX105433

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Sheep, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEF2C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

myocyte enhancer factor 2C , C5DELq14.3 , DEL5q14.3

Cellular Localization

Nucleus

Background

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MEF2C antibody used in IHC (Frozen sections) (IHC-Fr). GTX105433

GTX105433 IHC-Fr Image

MEF2C antibody detects MEF2C protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: MEF2C stained by MEF2C antibody (GTX105433) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MEF2C antibody used in Western Blot (WB). GTX105433

GTX105433 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX105433 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEF2C antibody used in Immunoprecipitation (IP). GTX105433

GTX105433 IP Image

MEF2C antibody immunoprecipitates MEF2C protein in IP experiments.
IP samples: K562 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of MEF2C protein by 4 μg MEF2C antibody (GTX105433)
5 % SDS-PAGE
The immunoprecipitated MEF2C protein was detected by MEF2C antibody (GTX105433) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433

GTX105433 IHC-P Image

MEF2C antibody detects MEF2C protein at nucleus on Saos2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Saos2 xenograft.
MEF2C antibody (GTX105433) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MEF2C antibody used in IHC (Paraffin sections) (IHC-P). GTX105433

GTX105433 IHC-P Image

MEF2C antibody detects MEF2C protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
MEF2C antibody (GTX105433) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MEF2C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105433

GTX105433 ICC/IF Image

MEF2C antibody detects MEF2C protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEF2C protein stained by MEF2C antibody (GTX105433) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169