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MEF2D antibody

Anti-MEF2D antibody used in IHC (Paraffin sections) (IHC-P). GTX02187
Anti-MEF2D antibody used in Western Blot (WB). GTX02187
Anti-MEF2D antibody used in Immunoprecipitation (IP). GTX02187
Anti-MEF2D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02187
Anti-MEF2D antibody used in IHC (Paraffin sections) (IHC-P). GTX02187

Cat No. GTX02187

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
IP 1-5 μl/mg of lysate
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Observed MW

70 kDa. The observed M.W. is based on the following publication. PMID: 28775333

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.0)

Preservative

0.03% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E. coli-derived Human MEF2D fragment

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myocyte enhancer factor 2D

Cellular Localization

Nucleus

Background

This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Database

Research Area

DATA IMAGES

Anti-MEF2D antibody used in IHC (Paraffin sections) (IHC-P). GTX02187

GTX02187 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX02187 MEF2D antibody.
Dilution : 1:100

Anti-MEF2D antibody used in Western Blot (WB). GTX02187

GTX02187 WB Image

WB analysis of various samples using GTX02187 MEF2D antibody.
Lane A : HeLa whole cell lysate
Lane B : Jurkat whole cell lysate
Lane C : U-251 MG whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-MEF2D antibody used in Immunoprecipitation (IP). GTX02187

GTX02187 IP Image

IP analysis of Jurkat whole cell lysate using GTX02187 MEF2D antibody.
IP antibody : 4 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
Immunomagnetic beads Protein A/G : 60 μg

Anti-MEF2D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02187

GTX02187 ICC/IF Image

ICC/IF analysis of PFA-fixed U2OS cells using GTX02187 MEF2D antibody.
Green : Primary antibody
Dilution : 1:200
Permeabilzation : 0.1% Triton X-100

Anti-MEF2D antibody used in IHC (Paraffin sections) (IHC-P). GTX02187

GTX02187 IHC-P Image

IHC-P analysis of human skeletal muscle tissue section using GTX02187 MEF2D antibody.
Dilution : 1:100

REFERENCE

There are currently no references for MEF2D antibody (GTX02187). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 339