GeneTex
United States (US)

MEFV antibody [N3C1], Internal

GTX106272_42823_20160420_WB_19011506_685.jpg
GTX106272_40632_IFA_19011506_502.jpg
GTX106272_42823_20170427_WB_19011506_484.jpg

Cat No. GTX106272

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

A431 , K562 , THP-1 , HL-60
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MEFV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mefv, Pyrin Innate Immunity Regulator,Fmf,Mef,Trim20,Mefv

Cellular Localization

Isoform 1: Cytoplasm , cytoskeleton , Isoform 2: Nucleus

Background

This gene encodes a protein, also known as pyrin or marenostrin, that is an important modulator of innate immunity. Mutations in this gene are associated with Mediterranean fever, a hereditary periodic fever syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106272_42823_20160420_WB_19011506_685.jpg

GTX106272 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MEFV antibody [N3C1], Internal (GTX106272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106272_40632_IFA_19011506_502.jpg

GTX106272 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MEFV(GTX106272) antibody at 1:500 dilution.

GTX106272_42823_20170427_WB_19011506_484.jpg

GTX106272 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MEFV antibody [N3C1], Internal (GTX106272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169