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MEK1/2 (phospho Ser218/222) antibody

Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX39720

Cat. No. GTX39720

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($659)
See all MEK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/1,000

Note :

WB
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result i
Not tested in other applications.

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Monkey, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

10mM HEPES pH7.5, 0.01% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated residues ser 218 and 222 within human MEK1 and 2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX39720

GTX39720 WB Image

WB analysis of NIH 3T3 cell lysate using GTX39720 MEK1/2 (phospho Ser218/222) antibody in serum starved (control) or incubated in the presence of serum (serum) for 5 minutes.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 659